Hello,

I was wondering why in transformation when calculating transformation efficiency do we divide by the *supercoiled **DNA*?

Transformation efficiency=Colonies/?g of *supercoiled **DNA*. **Is the (bigger)**recombinant plasmid **DNA more supercoiled** **than** the **(smaller)**non-recombinant plasmid DNA? Therefore, in that way we only calculate the recombinant plasmids in the denominator?

Thank you very much,